| abstract |
The present invention relates to a novel enzyme, mannanase and a fragment thereof, a nucleic acid molecule encoding the same, a recombinant vector comprising the nucleic acid molecule, a recombinant microorganism in which the recombinant vector is inserted into a host cell, A novel mannanase and fragments thereof according to the present invention have substrate specificity for guar gum and locust bean gum, and the other mannanases and fragments thereof have substrate specificity for guar gum and locust bean gum, In addition to having a high enzyme activity against guar gum in comparison to locust bean gum, and exhibits high reactivity at neutral pH and middle temperature, which are environments close to the intestines of animals, so that hydrolyzate can be efficiently obtained. Therefore, mannan and its fragments according to the present invention can effectively decompose guar gum. Mannose can be produced by this method, and the above mannanase or a fragment thereof can be added to the biomass to produce biomass And can be effectively utilized in various industrial fields. |