| abstract |
The present invention relates to a method for mass production of a therapeutic protein, comprising the steps of: (i) 5'-arm, which comprises a polynucleotide having a nucleotide sequence as set forth in SEQ ID NO: 1, , 4,840 bp fragment containing 5, 6 and 7; Ii) a polynucleotide encoding a therapeutic protein linked in frame to said beta-casein coding region; And iii) a genetic construct comprising a fragment comprising exon 8 and exon 9 of the beta-casein coding region of the bovine genomic DNA consisting of a polynucleotide having the nucleic acid sequence set forth in SEQ ID NO: 2 as the 3'- , Knock-in vectors for the production of foreign proteins in transgenic plants. |