| abstract |
The present invention relates to a method for producing porcine somatic cells in which a HNF6 (hepatocyte nuclease factor 6) gene targeting vector derived from a pig is knocked out, and a porcine somatic cell produced by knocking out the HNF6 gene produced by the above method HNF6 gene knock-out pig embryo, a method for producing a transformed pig in which the HNF6 gene is knocked out by using the embryo, and a transgenic pig produced by the method and knocking out the HNF6 gene. When the HNF6 gene knockout vector developed in the present invention is used, the HNF6 gene can be effectively knocked out in the target cell. Therefore, when the HNF6 gene is knocked out, It will be widely used in research to produce pigs. |