Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_259045c6eba6e2c5d338b84e0a88b571 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-02 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
filingDate |
2014-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03fdbfdceb9070956a2b131804cb003b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b3633841f3d2a4fb0f111a0cea22052 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5bfff31307b6b0733affff9f08fd29a4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9364a4c0549f0636181eee82ea441986 |
publicationDate |
2015-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
KR-20150143177-A |
titleOfInvention |
Hydrogel-in-oil emulsion containing cell, method for in vitro compartmentalization and ultra-high throughput screening of novel enzyme |
abstract |
The present invention relates to hydrogel-in-oil emulsions containing cells, an in vitro compartmentalization (IVC) method, and an ultra-high throughput screening (uHTS) method for novel enzymes. The present invention can prevent the diffusion of a product generated by a reaction between cells and a substrate in hydrogel to enhance the fluorescence intensity, thereby being able to be utilized for a fluorescence-activated cell sorting (FACS) analysis. According to the present invention, the emulsions prepared by using the IVC method are stable and are able to be stored for an extended period. The emulsions can be used to culture cells and enable a single cell to proliferate to two or three cells. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102087928-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102087927-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20210056132-A |
priorityDate |
2014-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |