| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2a819eda0adf22936a52362eeebb9fb4 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2440-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-435
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2035-1034
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2035-00237
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N35-1081
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N35-1072
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N35-1016
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M47-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-0237
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01Q60-44 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N35-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate |
2014-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_34b95e569745a0ea2e0309a9eda0675d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d22659a7a05bfb5f33c9813318c519c8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91e99b835cadc712e3651e4fc927a823 |
| publicationDate |
2015-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
KR-20150128864-A |
| titleOfInvention |
Nanopipette device and method for subcellular analysis |
| abstract |
The present invention describes an apparatus and a method on a nanoliter scale for extracting cellular material from living cells and then placing it in a container. Extraction of mitochondrial DNA from human BJ fibroblasts and green fluorescent protein (GFP) transcripts from HeLa / GFP cells using a nanopipette integrated with a scanning ion-conducting microscope (SICM) And without chemical treatment prior to obtaining the isolated sample. The success of this extraction was confirmed by fluorescence microscopy and PCR analysis of the extracted material. The methods and devices can be applied to many different cell types and intracellular targets and enable single intracellular compartment analysis of naturally extracted material as well as single cell analysis. |
| priorityDate |
2013-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |