| abstract |
In particular, there is provided a method of stabilizing a eukaryotic cell line expressing PylRS and tRNAPyl, which is suitable for incorporation of a gene encoding a target protein containing at least one unnatural amino acid encoded by a nonsense codon, wherein the tRNAPyl expression is indicative of cell viability and / Culturing the cell line under conditions such that a negative effect on growth is reduced or eliminated. |