http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20150049041-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7bb83893a72280b4b778e0e3262c97f8 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0653 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0607 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
filingDate | 2013-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_382118d412029322f63916e59209b706 |
publicationDate | 2015-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-20150049041-A |
titleOfInvention | Method for culturing adiposed derived stem cell |
abstract | The present invention relates to a method for culturing adipose-derived stem cells isolated from a human body. The method for culturing adipose derived stem cells according to the present invention is a method for culturing adipose derived stem cells isolated from adipose tissue of a human under hypoxia conditions in which oxygen content is lower than atmospheric conditions through a primary culture stage and a subculture culture stage , In the primary culture step, the first culture medium is prepared by inoculating and culturing stem cells into a first culture medium comprising a liquid nutrient medium containing glucose, plasma separated from the human body, platelet rich plasma irradiated with visible light, and a first additive , The subculturing step is characterized in that the second culture medium containing the nutrient medium and the bovine serum and the second additive mixed at a ratio of 1 to 5 wt% based on the weight of the nutrient medium is inoculated with the stem cells through the primary culture step . |
priorityDate | 2013-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 178.