| abstract |
The present invention discloses polynucleotides comprising at least the last six nucleotides of one of the primer sequences of SEQ ID NOS: 3 to 16, 18, 20 to 33, 35 or 37 to 39, or a sequence complementary thereto. Also disclosed is a method of detecting the presence of a mutation in a PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, preferably the ARMS primer is combined with a scorpion primer. |