http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20150005895-A

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filingDate 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c8f4f55b7cc9c0e6f42d954803368d2
publicationDate 2015-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-20150005895-A
titleOfInvention Efficient Multiplex PCR method using fluorescent markers and mobility markers
abstract The present invention relates to an efficient multiplex PCR method using a fluorescent labeling substance and a mobile marker. More specifically, in order to attempt two or more PCRs having different targets in one reaction solution, two or more primer sets are used, In the multiplex PCR in which the above-mentioned primers are labeled with different labeling substances according to the respective primer sets to distinguish the PCR amplification products according to the target, a combination of 6-FAM, JOE, ALEXA 546 and ALEXA 568 Lt; RTI ID = 0.0 &gt; PCR &lt; / RTI &gt; According to the present invention, the PCR amplification products for each target can be clearly identified even in the multiplex PCR of four or more targets, and it is possible to detect multiple targets such as genetic testing, identification of organisms in the sample, If it is necessary to confirm the presence of DNA or RNA, accurate results can be obtained quickly and easily. In particular, even when a short repeated sequence is targeted, amplification can be effectively confirmed.
priorityDate 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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