| abstract |
The present invention non-transgenic malto exo-mutant as derived from the PS4 parent polypeptide having a-amylase activity of the polypeptide, wherein the mutation PS4 polypeptide is SEQ ID NO: pilriah (Pseudomonas as Pseudomonas saccharide represented by 1 (K) or arginine (R) of residue 307 from a saccharophilia exo-amylase sequence. Preferably the PS4 variant polypeptide further comprises an amino acid substitution at residue 70, preferably G70D. The amino acids at residues 272 and 303 of the amino acid sequence are preferably histidine (H) and glycine (G). |