| abstract |
Identification of new enhancer sequences is significantly useful in plant functional genomics. A transcription enhancer of sugar cane Bacillus-type bard virus (SCBV) was identified. Such enhancers can be used to increase the rate of transcription from a gene promoter, and in an activation tagging experiment. A 10-fold increase in transcription was observed when a 4x array of SCBV enhancers was placed upstream of the truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer, and chimeric transcriptional regulatory regions, constructs, cells, tissues, and organisms comprising one or more copies of such enhancers are described. |