http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20140129841-A

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inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_34a343cd217d7a23aab4efa215c48038
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publicationDate 2014-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-20140129841-A
titleOfInvention A screening method for the optimal surface structure of embryonic stem cell culture using a cell culture plate comprising gradient nanopattern
abstract The present invention provides a method for screening a surface structure suitable for embryonic stem cell culture comprising culturing a cell in a cell culture container containing a gradient nano pillar array having a constant diameter or diameter, A cell culture container containing nano pillars uniformly arranged at intervals such that the nano pillars having a constantly increasing diameter used in the screening method are uniformly arranged at an interval decreasing uniformly, and a cell culture comprising nanoparticles of the conditions selected by the screening method And culturing the embryonic stem cells while maintaining stem cell morphology without supporting cells comprising culturing the cells in a container. A cell culture container comprising nano pillars uniformly arranged in a uniformly decreasing spacing of nano pillars having uniformly increasing diameters of the present invention provides various surface morphologies in one culture container Therefore, it is possible to simultaneously monitor the effect of changes in the surface morphology on the cell characteristics by the single culture performed under the same conditions, and the optimum surface morphology suitable for cell culture can be selected using the same. The method of culturing the selected human embryonic stem cells through the screening can provide a method capable of proliferating while maintaining the stem cell function without coating the costly extracellular matrix or using the supporting cells.
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