| abstract |
The present invention provides a nucleic acid sequence encoding or encoding at least one peptide or protein comprising a therapeutic protein, fragment, variant or derivative thereof, at least one histone stem-loop and poly (A) sequence or polyadenylation signal, . In addition, the present invention also provides the use of said nucleic acid for increasing the expression of said encoded peptide or protein, particularly in gene therapy. The invention also relates to the use of said nucleic acid in the manufacture of a pharmaceutical composition, for example a gene therapy, in particular a pharmaceutical composition for use in the treatment of a disease to be treated with a therapeutic peptide or protein (preferably a therapeutic peptide or protein as defined herein) . The present invention also provides a method of increasing the expression of a peptide or protein comprising a therapeutic protein, fragment, variant or derivative thereof, using a nucleic acid containing or encoding a histone stem-loop and a poly (A) sequence or a polyadenylation signal Methods have also been described |