abstract |
The present invention provides a method for the production of isobutyric acid, comprising the steps of: a) providing isobutyric acid, b) combining isobutyric acid with a combination of isobutyrate kinase and phosphotrans isobutyrylase and / or isobutyryl-coenzyme A synthetase / Isobutyrate-coenzyme A transferase, c) contacting the product from step a) with isobutyryl-coenzyme A dehydrogenase, d) contacting the product from step b) with a methacryloyl-coenzyme A hydratase, and e) hydrolyzing the product from step d) to form 3-hydroxyisobutyric acid. One or more of the above enzymes are used in the form of a cell containing the activity of 3-hydroxyisobutyric acid dehydrogenase or a variant thereof whose activity is reduced as compared with the wild type of the cell. The present invention also relates to the use of isobutyryl-coenzyme A synthetase / ligase, isobutyrate-coenzyme A transferase, isobutyrate kinase, phosphotrans isobutyrylase, isobutyryl-coenzyme A dehydrogenase, methacryloyl - 3-hydroxyisobutyric acid, which contains at least one enzyme selected from the group consisting of coenzyme A hydratase and 3-hydroxyisobutyryl-coenzyme A hydrolase and whose activity is reduced compared to wild type of cells Dehydrogenase, or a variant thereof. In addition, the cell preferably has a monooxygenase, more preferably a monooxygenase of the AlkBGT type or a variant thereof. The present invention also relates to the use of said cells for producing 3-hydroxyisobutyric acid. |