http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20140101890-A

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filingDate 2012-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4689c7141a211681be13478441d9be46
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1a6b699b0e08f8dc0adc905da42d804
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publicationDate 2014-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-20140101890-A
titleOfInvention Biotechnological production of 3-hydroxyisobutyric acid
abstract The present invention provides a method for the production of isobutyric acid, comprising the steps of: a) providing isobutyric acid, b) combining isobutyric acid with a combination of isobutyrate kinase and phosphotrans isobutyrylase and / or isobutyryl-coenzyme A synthetase / Isobutyrate-coenzyme A transferase, c) contacting the product from step a) with isobutyryl-coenzyme A dehydrogenase, d) contacting the product from step b) with a methacryloyl-coenzyme A hydratase, and e) hydrolyzing the product from step d) to form 3-hydroxyisobutyric acid. One or more of the above enzymes are used in the form of a cell containing the activity of 3-hydroxyisobutyric acid dehydrogenase or a variant thereof whose activity is reduced as compared with the wild type of the cell. The present invention also relates to the use of isobutyryl-coenzyme A synthetase / ligase, isobutyrate-coenzyme A transferase, isobutyrate kinase, phosphotrans isobutyrylase, isobutyryl-coenzyme A dehydrogenase, methacryloyl - 3-hydroxyisobutyric acid, which contains at least one enzyme selected from the group consisting of coenzyme A hydratase and 3-hydroxyisobutyryl-coenzyme A hydrolase and whose activity is reduced compared to wild type of cells Dehydrogenase, or a variant thereof. In addition, the cell preferably has a monooxygenase, more preferably a monooxygenase of the AlkBGT type or a variant thereof. The present invention also relates to the use of said cells for producing 3-hydroxyisobutyric acid.
priorityDate 2011-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ17334
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ24641
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA6ZTT5
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP43067
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP23278
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCO46650
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ17335
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ03505
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP23237
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID798364
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP12711
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ8NXU1
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP49645
http://rdf.ncbi.nlm.nih.gov/pubchem/taxonomy/TAXID1270
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ0DWH1
http://rdf.ncbi.nlm.nih.gov/pubchem/taxonomy/TAXID303
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP10847
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ05114
http://rdf.ncbi.nlm.nih.gov/pubchem/taxonomy/TAXID9627
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ00671
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP10848
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCA0A165U5V5
http://rdf.ncbi.nlm.nih.gov/pubchem/taxonomy/TAXID318829
http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID410627548
http://rdf.ncbi.nlm.nih.gov/pubchem/taxonomy/TAXID2587410
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ00672
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP54202
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP26719
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ00669
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID100037216
http://rdf.ncbi.nlm.nih.gov/pubchem/gene/GID548942
http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID165390
http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419527785
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP11766
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCQ00670
http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP0DJA2

Total number of triples: 335.