| abstract |
The present invention relates to a method for producing a thrombolytic enzyme using liquid fermentation of a pupa of Cordyceps sinensis, which comprises: (1) cultivating a pupa of Cordyceps sinensis strain in a PD liquid fermentation medium, activating the culture, Proceed to obtain seed solution; (2) The seed liquid is inoculated into the liquid fermentation medium at a volume ratio of 5 to 15%, the liquid fermentation is carried out for 20 to 30 hours, the expansin solution is added and the culture is continued for 120 to 168 hours, Isolating and removing the mycelia of the pupae of Cordyceps sinensis to obtain a thrombolytic enzyme fermentation supernatant; (3) Extract from the fermentation supernatant of the thrombus lysis enzyme Extract the insect pupae fibrinolytic enzyme which is the extracellular thrombolytic enzyme of the pupa. The present invention is an application of plant expansin and an optimized fermentation method to the production of liquid fermentation of pupa chrysanthemum fibrinolytic enzyme, and the liquid fermentation yield of the pupa chinensis thrombosis enzyme is pulled up to 93280 U per 1 liter, It has a considerable prospect in terms of industrial application. |