| abstract |
The present invention relates to a method for producing chordysepinic acid using liquid fermentation of a pupa of Cordyceps sinensis, which comprises: (1) cultivating a puddied Cordyceps sinensis strain in a PD liquid fermentation medium, activating the culture, transferring it to a seed fermentation medium, Proceed to obtain seed solution; (2) The seed liquid is inoculated into a liquid fermentation medium at a volume ratio of 5 to 15%, followed by liquid fermentation for 20 to 30 hours, then expansin solution is added, and the culture is continued for 96 to 144 hours, To obtain a pupae mycelium of pupa; (3) Cordycepinic acid in the pupae of Cordyceps sinensis cells is obtained from the mycelia of pupae. The production of chondisipinic acid fermentation can be improved by using expansin for the production of liquid fermentation of cordisepinic acid in the pupa of cordyceps. |