| abstract |
The present invention relates to a method for the production of proteins, for example, free-cells containing about 5% malt extract to express proteins that are assembled into a multiprotein complex in a manner similar to viral proteins and proteins required for virus capsid assembly, System. Using a cell-free system containing about 5% malt extract, proteins, such as viral proteins, proteins required for viral capsid assembly, and proteins assembled into a multiprotein complex in a manner similar to virus capsids, Lt; / RTI > There are further provided assay methods for virus proteins, virus capsid assemblies, and compounds that modulate the assembly of proteins into a multiprotein complex, wherein the cleavage of the assembly is carried out in a cell-free system comprising less than about 5% malt extract Bacterial, parasitic, metabolic, tumor, immunological, or CNS disorders. |