http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20140064496-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3f4508d36cd90890103c299ac719aada |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-363 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-30 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-27 |
| filingDate | 2012-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e417ee43dc43f6b25d8040c20a37152 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0496a7a5a6e37f93919b7bc0109b0dc6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7279466183ab249d8c03dcd04e44a00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1947eec93e751cbda145423f1fa648ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db66a757e7b324fc140f56d563f36887 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7be78136468ffa0426dbfc21d1eb310 |
| publicationDate | 2014-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20140064496-A |
| titleOfInvention | IGF-I Isolation Method from Cow Colostrum |
| abstract | The present invention provides a method for isolating insulin-like growth factor 1 (IGF-I) from mammalian milk comprising the steps of: (a) separating mammalian milk into whey and casein; (b) acidifying the whey of step (a) to pH 1-4 and then centrifuging to recover a fraction containing a large amount of IGF-I; (c) fractionating a fraction containing a large amount of IGF-I in the step (b) by ion exchange chromatography using an anion exchange resin to obtain a fraction containing a large amount of a substance corresponding to the molecular weight of IGF- Separating; (d) separating a fraction containing a large amount of a substance corresponding to the molecular weight of IGF-I of the step (c) by using a gel filtration chromatography; And (e) passing a fraction containing a large amount of IGF-I in step (d) through a filtration membrane of 7000-13000 Da to recover a fraction of 7000-13000 Da or less, and concentrating the filtrate to 700-1300 Da to recover IGF- step. The method of the present invention provides the advantage that the separation and purification process is significantly simpler than the previously reported IGF-I separation and purification method (Francis et al., 1986). In addition, the method of the present invention has an advantage that the content of IGF-I recovery can be increased three times or more as compared with the previously reported IGF-I separation and purification method. |
| priorityDate | 2012-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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