http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20140057493-A

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filingDate 2012-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_583ceb82a5157d89e19bea093c326af9
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publicationDate 2014-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-20140057493-A
titleOfInvention Agents and methods for passage and obtaining of human pluripotent stem cells
abstract The present invention relates to agents and methods for obtaining and passing human pluripotent stem cells that separate cells from cell culture vessels without using enzymes and / or scraping. The formulation and method of the present invention makes it possible to obtain cells with large clusters from the surface of various cell culture vessels including multilayer cell culture vessels. Furthermore, the preparations and methods of the present invention provide high cell yields in the passage and high cell viability after harvest. According to the method of the present invention, the pluripotent stem cells remain undifferentiated using the preparation of the present invention, and express stem cell markers. Also, it has the ability to differentiate at the same time, and it can be all differentiated into three germ layers even after getting through and passing through several times, and teratomas can be produced. These human pluripotent stem cells exhibit a normal karyotype even after the administration using the agent of the present invention for an extended period of time.
priorityDate 2011-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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