abstract |
The present invention relates to methods for producing breast milk oligosaccharides (HMOs) or precursors thereof, compounds obtainable by the above methods, and uses and compositions comprising such compounds. The method comprises a) providing at least one donor selected from the group of any compound of formulas 5-10, b) providing at least one acceptor from the group of lactose, LNT, LNnT and derivatives thereof. C) providing at least one enzyme comprising transglycosidase activity and / or glycosinase activity; d) preparing a mixture of at least one donor, at least one recipient and at least one enzyme provided in steps a), b) and c); And e) incubating the mixture prepared according to step d). |