http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20140030429-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2b4be2300a741a69aa1b8bb47a88899f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-045 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2012-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2ccf02107c4d068573df297068db5f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30911cce27e4110035dda95878cd21a5 |
| publicationDate | 2014-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20140030429-A |
| titleOfInvention | Detection of KPC and MBL production by liquid medium microdilution method using β-lactamase inhibitor |
| abstract | The present invention relates to a method for detecting the production of Klebsiella pneumoniae kappa penemezes (KPCs) and metallo-beta-lactamases (MBLs) in clinical isolates of Gram negative bacilli using various beta-lactamase inhibitors Dilution method. The carbapenemase detection test method includes a sequential double dilution of imipenem or meropenem, and includes a solution of aminophenylboronic acid (APB), phenylboronic acid (PB), clocksulfuric acid (CLX) (KPC), 21 MBL producing strains and porin were lost, and plasmid AmpC production or chromosomal aberrations were detected. AmpC < / RTI > overexpression was evaluated against 16 Enterobacteriaceae strains. The minimum inhibitory concentration of imipenem or meropenem in the presence of? -Lactamase inhibitor was reduced by 3-fold or more as compared with the case of treatment with imipenem or meropenem alone, and this result was judged as a positive result. The results obtained using the liquid medium microdilution method of the present invention with imipenem with or without phenylboronic acid, including the additional criteria of negative CLX results in the detection of Klebsiella pneumoniae, provide the most comparable sensitivity to KPC enzymes (94%) and specificity (95%). Both DPA and EDTA have excellent sensitivity to detect MBL producing gram negative bacilli (90% versus 95% for imipenem treatment with or without DPA vs. EDTA; 95% versus 95% for meropenem treatment with or without DPA vs. EDTA) %) And specificity (98% versus 100% for imipenem treatment with or without DPA vs. EDTA; 98% vs 98% for treatment with meropenem with or without DPA vs. EDTA). The present invention showed that a liquid medium microdilution test using imipenem with or without phenyl boronic acid, clock factin and EDTA provided the most effective results for KPC and MBL enzyme detection. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114002429-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114002429-A |
| priorityDate | 2012-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.