| abstract |
The method includes fixing a bead carrying a biological material (e.g., a nucleic acid that may be in the form of a DNA fragment or amplified DNA) to a specific location on the substrate by a magnetic force, and applying a localized electric field to the bead Isolating the reaction product or by-product of the substance or biological material. For example, the beads are isolated from other beads associated with the biomaterial. In various embodiments, the electric field enriches and / or enriches the reagents for amplification or sequencing reactions, or enriches and isolates detectable reaction by-products. For example, by isolating the nucleic acid around the individual beads, the electric field can allow for clone amplification as an alternative to emulsion PCR. In other embodiments, the electric field isolates the nanosensors that are close to the beads to facilitate detection of one or more of local pH changes, local conductivity changes, local charge concentration changes, and localized fever. The beads can be collected in the form of a localized magnetic field area array. |