| abstract |
Disclosed are a method and a system for analyzing multiplexing treatment of a target material using gold nanoparticles to improve signal amplification. The analysis system of this target material includes a support, a capturer, gold nanoparticle complex, a fluorescent RNA detector, and ribonuclease H, which is fixed to the support and selectively binds to the target material. In this complex, the detector binds to the surface of the gold nanoparticles as a different material from the trapper, selectively binding to the target material. In the other region of the surface of the gold nanoparticle, a plurality of single strands of the same deoxyribonucleotide are connected. The fluorescent RNA detector is unique to the detector-gold nanoparticle complex and comprises a single stranded ribonucleotide linked to a fluorescent source and a quencher for the fluorescent source at the end, the ribonucleotide complementary to the deoxyribonucleotide Inclusive sequence. When the target material of the trapper and the detector is present in the sample, hybridization of the deoxyribonucleotide and the ribonucleotide occurs, and the ribonuclease H is hydrolyzed to release fluorescence inhibition to generate and amplify a fluorescence signal. |