| abstract |
The present invention relates to denitrification of municipal sewage under anoxic and aerobic conditions, and more particularly, Pseudomonas sp. LOCJN5 (deposited heterotrophic denitrifying bacteria isolated from a continuous reactor for removing nitrogen, phosphorus and organic matter). No. KCTC 12098BP) and Pseudomonas genus LOCJN5 to denitrification of domestic sewage. The strain of the present invention is 99.92% similar to Pseudomonas aeruginosa LMG 1242T in comparison with ssurRNA gene sequence homology, and is a novel bacteria Pseudomonas genus LOCJN5 which is closely related to other standard strains of Pseudomonas genus. I was identified. Pseudomonas genus LOCJN5 of the present invention is a Gram-negative bacillus having catalase and oxidase activity, and has the activity of esterase (C4), esterase lipase (C8), lipase (C14), leucinearylamidase, trypsin and acid phosphatase, D-glucose, L -arabinose, D-mannose, D-mannitol, N-acetyl-glucosamine, potassium gluconate, capric acid, adipic acid, malic acid and trisodium citrate as carbon sources, ammonia, nitrates, nitrites as nitrogen sources, nitrates Can be reduced to nitrogen. Pseudomonas genus LOCJN5 of the present invention is grown in a medium containing ammonia nitrogen, nitrate nitrogen or nitrite nitrogen as the only nitrogen source and can remove nitrogen, Pseudomonas genus LOCJN5 is 500 mg / L quality contained in artificial sludge Acidic nitrogen was reduced to 0 mg / L in anoxic conditions and to 88 mg / L in aerobic conditions. |