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publicationDate 2013-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-20130076892-A
titleOfInvention Highly sensitive method of determining the quantity of herbal medicine-derived components
abstract The high sensitivity quantification method of the herbal-derived component is injected into a solid phase having a reverse phase distribution function and an anion exchange function by incorporating a blend of a biological sample into an alkali or an alcohol, and then introducing the solid phase into water, an alkali, an alcohol, and acetonitrile. An extraction step of preparing an extract containing a crude drug-derived component by washing at least once with one or two or more kinds of mixed liquids selected from the group consisting of, and then eluting from the solid phase with an acidic alcohol, and the extraction step At least one selected from the group consisting of glycyrrhizin, glycyrrhizin acid, glycyrrhizine and metabolites of glycyrrhinic acid, flexible substances of glycyrrhizine and glycyrrhinic acid, saponin components in licorice, and pharmaceutically acceptable salts thereof in the extract extracted by It includes the step of detecting and quantifying one species by mass spectrometry.
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