| abstract |
Described herein are immunostimulatory compositions and methods comprising an ITIM motif-containing DC immunoreceptor (DCIR) to mediate strong cross presentation. We evaluated human CD8 + T cell responses generated by targeting antigens to dendritic cells (DCs) through various lectin receptors. Single exposure to low doses of anti-DCIR-antigen conjugates resulted in antigen-specific CD8 + T cell immunity by ex vivo DCs, skin-separated Langerhans cells and all human DC subsets including blood mDCs and pDCs. Started. Antigens such as FluMP, MART-1, virus (HIV gag), etc., are delivered to DC via DCIR, compared to when induced by free antigen or antigen conjugated to control mAb or via DC-SIGN, another lectin receptor Enhanced specific CD8 + T cell responses are observed. The addition of toll type receptor (TLR) 7 / 8-agonists enhanced crosspriming as well as DCIR-mediated crosspresentation. Thus, antigen targeting via human DCIR receptors enables activation of specific CD8 + T cell immunity. |