| abstract |
The present invention relates to a method for proliferating BHK-21 cells using sex-specific serum, and a method for increasing the production yield of viral vaccines such as foot-and-mouth disease using the BHK-21 cells, and more specifically, sex-specific serum, especially males. BHK-21 cells can be efficiently grown using serum or castration male serum, which is superior to fetal fetal serum (FBS) by using sex-specific serum at a lower concentration than conventional fetal calf serum (FBS). Since it can be induced, it can be usefully used for reducing the cost of cell proliferation, reducing the environmental burden of carcass waste, and increasing profits due to high quality serum production. In addition, since the cell proliferation effect is significantly increased in BHK-21 cells compared to other cell lines such as Vero cells, the foot-and-mouth disease vaccine is cheaper and more effective using BHK-21 cells cultured with sex-specific serum. Can produce. |