| abstract |
The present invention provides spr having mutations in at least one amino acid selected from D133, H145, H157, N31, R62, I70, Q73, C94, S95, V98, Q99, R100, L108, Y115, V135, L136, G140, R144 and G147. It provides a recombinant Gram-negative bacterial cell comprising a mutant spr gene encoding a protein and having reduced Tsp protein activity compared to wild-type cells. |