| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a16f72268f5a1eb4f08073323a474668 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-327 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1276
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-26004
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07007
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07049
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate |
2011-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_37269e224a364f4f65aefdd21cf0e8ff
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15167b6ebf3dfe0f73c57d594b777b91 |
| publicationDate |
2013-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
KR-20130021351-A |
| titleOfInvention |
Detection of Modified RNA NS and Nucleic Acid Amplification |
| abstract |
Disclosed are 'hot start' RNAse H enzyme compositions that are reversibly modified for improved CATACLEAVE ™ probe detection of nucleic acid sequences in test samples. An important feature of the enzyme composition is the ability to modulate the catalytic activity of RNAse H during reverse transcription-PCR reactions. Thus, RNAse H activity can be initially inhibited to minimize degradation of RNA: DNA primer heteroduplex prior to reverse transcription. After cDNA synthesis is complete, CATACLEAVE ™ induces RNAse H activity to anneal to target DNA sequences in reverse transcriptase-PCR products Promote cleavage and fluorescence detection of the probe. The inducible RNAse H enzyme is a one-step reverse transcriptase CATACLEAVE ™ in a single reaction mixture. It is suitable for high-speed mass analysis requiring PCR. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20210035066-A |
| priorityDate |
2010-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |