| abstract |
The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single stranded mRNA encoding iPS cell inducers. Said purified RNA preparation is preferably substantially free of RNA contaminant molecules, i) capable of activating an immune response in somatic cells, ii) reducing the expression of single stranded mRNA in somatic cells, and / or iii) It may be possible to activate RNA sensors in somatic cells. In some embodiments, the purified RNA preparation is substantially free of partial mRNAs, double stranded RNAs, uncapped RNA molecules, and / or single stranded run-on mRNAs. |