| abstract |
The present invention provides a method for producing fetal mesenchymal stem cells in amniotic fluid comprising the step of culturing a cell proliferation accelerator comprising selenium as an active ingredient, amniotic fluid-derived cells in FBS (fetal bovine serum) and selenium-containing medium, Amniotic fluid-derived mesenchymal stem cells prepared by the above method, a medium composition for proliferating mesenchymal stem cells containing FBS and selenium, a method for preparing a culture solution of the amniotic fluid-derived mesenchymal stem cells, and prepared by the method The present invention relates to a cosmetic composition for improving skin condition, a pharmaceutical composition, and a method for mass production of proteins related to wound treatment. By providing a fetal mesenchymal stem cells in amniotic fluid cultured in a medium containing commercially available selenium in place of the expensive cytokine bFGF according to the present invention, while lowering the production cost of mesenchymal stem cells, addition of selenium or bFGF And it is possible to provide mesenchymal stem cells having an excellent effect through the co-addition of selenium, it is possible to provide a composition excellent in cell growth and skin regeneration ability using the culture of the mesenchymal stem cells. |