| abstract |
The present invention relates to a high-purification method of hyaluronidase and an injection using the same, (a) preparing a raw material through lyophilized hyaluronidase after extraction from ovine testis, and (b) A first step of purifying the hyaluronidase prepared in step (a) with Blue sepharose (Blue sepharose), the step (b) provides a configuration comprising the step of washing with sodium caprylate. By using the above-described high purification method of hyaluronidase and injection using the same, it is possible to increase the purity of hyaluronidase commercialized previously, and to remove foreign matter protein, which is a problem of hyaluronidase commercialized. By doing so, safety can be ensured. |