http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20120105640-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e5351c62aee4203f2be2944f21a6def2 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2011-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5997c8a3c23e6df921290c3bb7610d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_42d038c94bd076e4334335121efcfb99 |
| publicationDate | 2012-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20120105640-A |
| titleOfInvention | Assembly method into a single nucleic acid sequence of multiple target positions |
| abstract | The present invention relates to a method for assembling multiple target loci into one assembled nucleic acid sequence, and a method for simultaneous detection using the same. The method of the present invention assembles multiple target positions into one assembled nucleic acid sequence through two PCRs, a first polymerase chain reaction and a second PCR reaction. More specifically, the primary pair of amplification primers (forward primer and reverse primer) used in the present invention is a target-specific sequence (target hybridizing nucleotide sequence) and a 5'- flanking assembly spacer sequence (overlapping sequence). consist of. In addition, the first amplification product amplified using the first amplification primer pair can be easily and easily assembled into one shortened nucleic acid sequence through a second amplification primer set to simultaneously detect multiple target positions. Can be. Thus, the methods and kits of the present invention can significantly reduce the sequencing cost for variant detection by simultaneously detecting and analyzing multiple variants (eg, SNPs) on the DNA sequence of a sample (preferably human blood). In addition, it provides an important approach and means to realize the concept of personalized medicine. |
| priorityDate | 2011-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 139.