http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20120052872-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_259045c6eba6e2c5d338b84e0a88b571 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-21 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 |
| filingDate | 2011-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07327c33ce52dc8564b122a8c1039f53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4b8fc6365dfbdb4f7e6eddd644897e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_65286349b8ae8c814318ec9dd3834ebb |
| publicationDate | 2012-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20120052872-A |
| titleOfInvention | Red fluorescent protein fragment having self-binding activity, preparation method thereof and analysis method of protein interaction using same |
| abstract | The present invention relates to a red fluorescent protein fragment having self-binding activity, a method for preparing the same, and a method for analyzing a protein using the same. The red fluorescent protein fragment (mPlum 1 E16V) according to the present invention connects the β-strand 1 of the red fluorescent protein (mPlum) through the flow peptide linker behind the β-strand 2-11, and the permutated mPlum protein (CpmPlum) After the preparation, the mutant (CpmPlum E16V) was prepared by substituting Val (valine) for 16 Glu (glutamic acid) in the amino acid sequence of β-strand 1 of the ordered mPlum protein (CpmPlum) and mutating alone. MPlum 1 peptide (mPlum 1 E16V) is isolated from mPlum 2-11 protein and self-binds with mPlum 2-11 protein to emit red fluorescent signal, thereby having self-binding activity. Thus, the red fluorescent protein fragment (mPlum 1 E16V) with self-binding activity according to the present invention can be used as a biosensor as a useful tool for analyzing protein function such as protein location in living cells, protein-protein interactions, etc. Can be. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20200085159-A |
| priorityDate | 2010-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 341.