http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20110128033-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1d1750b578ca9a780678f6b9b9c8b87d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2437 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-00 |
| filingDate | 2010-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_357bf0c41a4b96fcd48b92862e55995a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da1486fce67a633d7ff46d4047b62a80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af253dbbe32271398f840447a547a72a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00750b2408af8b15da34fefb2d2b6476 |
| publicationDate | 2011-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20110128033-A |
| titleOfInvention | Porodiculus pendurus-derived sugar degrading enzyme and biofuel production method using the same |
| abstract | The present invention relates to a glycolytic enzyme derived from Porodisculus pendulus and a method for producing biofuels by glycosylating biomass using the same. The optimal culture conditions for the production of P. pendulus glycolytic enzymes and the saccharification characteristics of woody biomass were studied. P. pendulus extracellular enzymes contained cellulase such as EG, BGL and CBH and BXL. Among them, EG and BGL activity was found to be relatively high. The optimum temperature of EG, BGL, and CBH produced in P. pendulus was 50 ℃, but the temperature range of thermal stability was 30 ~ 40 ℃. The optimum pH was 5.5 and the optimum pH in the temperature range indicating thermal stability was the same pH 5.5. Optimal culture conditions of P. pendulus for cellulase production were found to be suitable for increasing cellulase enzyme activity by adding natural cellulose as a carbon source, corn leach powder or peptone / yeast extract mixture as a carbon source, and no vitamin. In addition, the optimum concentration of carbon source was found to be 2% (w / w), and the optimum culture pH and temperature were 5.5-6.0 and 25-30 ° C. The optimum culture conditions derived from this study were cultured and concentrated 40-fold in P. pendulus.The results showed that EG was 3670.5 U / ml, BGL and CBH were 631.9 U / ml, 398.5 U / ml, and BXL 15.2 U / ml, respectively. Very high enzymatic activity. The filter paper unit of the same enzyme was also significantly higher at 11 FPU / ml. P. pendulus obtained under optimal culture conditions The saccharification test of various substrates with enzyme showed low glycosylation rate against untreated pre-treated hardwoods, but high saccharification of 50.5% and 33.1% for natural cellulose (Aldrich, ˜20 micron) and rice straw, respectively. Indicated. This glycosylation level represents about 110% of Triucderma reesei- derived commercial enzyme Celluclast 1.5L when compared to the same enzyme concentration (30 FPU / g, glucan). The strain was evaluated as having a high commercial potential. |
| priorityDate | 2010-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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