http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20110092225-A

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filingDate 2011-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9004458bf18a8424cdadced71634b951
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca60a495b2f8fc37bb8008b6e5242087
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http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_32503a9c5056bd16992492e22b1da8e8
publicationDate 2011-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-20110092225-A
titleOfInvention Method for producing a recombinant glycoprotein with high sialic acid content
abstract The present invention relates to a method for producing a recombinant glycoprotein having a high sialic acid content, and more particularly, arginine at amino acid 263 with leucine or arginine at amino acid 263 with leucine and arginine at amino acid 266. The UDP-GlcNAc 2-epimerase / ManNAc kinase (GNE / MNK) enzyme, which was substituted with glutamine or tryptophan to induce point mutations, was used to determine epimerase (regardless of the concentration of cytidine monophosphate (CMP) -sialic acid). epimerase activity is sustained and cells overexpressed increase the content of CMP-sialic acid in the cells, and in particular, GNE / MNK, human alpha-2,3 sialic acid sugar transferase, Intracellular CMP-sialic acid and glycoproteins in glycoprotein (erythropoietin or thrombopoietin) producing host cells simultaneously overexpressing the CMP-sialic acid transport protein gene Since the sialic acid content of the vagina is increased, overexpression in recombinant glycoprotein-producing host cells with sialic acid binding of the three genes can be useful for the preparation of glycoproteins with increased sialic acid content.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20160127124-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018030738-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015194704-A1
priorityDate 2010-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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Total number of triples: 873.