| abstract |
Described herein are methods of isolation and purification of antibodies using affinity chromatography steps that yield antibody compositions sufficiently pure for use in pharmaceutical use. The methods described herein include pH virus reduction / inactivation, ultrafiltration / diafiltration, affinity chromatography, preferably protein A affinity, ion exchange chromatography, and hydrophobic chromatography. The invention also relates to pharmaceutical compositions comprising one or more antibodies of the invention. |