http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20110050327-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_14430ce0c28e36044927f5834e4d02ad |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2561-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2561-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-1015 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2009-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7774a5ee918adc16e0bd95723bde4b3e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_34ac7f5c13a90df324db97c714067048 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_120d223f37d6893c0f2924f518c3fc54 |
| publicationDate | 2011-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20110050327-A |
| titleOfInvention | THD primer target detection |
| abstract | The present invention relates to a method and kit for detecting a target nucleic acid sequence in a DNA or nucleic acid mixture using a 5′-cleavage and 3′-extension reaction of a target hybridization and detection primer (THD primer). The present invention is characterized by using a THD primer having a dual function of providing a signal capable of detecting a target nucleic acid sequence as well as synthesizing a base sequence complementary to the target nucleic acid sequence. When the THD primers of the present invention are used in real-time PCR, not only amplification of the target nucleic acid sequence accompanied by an extension reaction of the reverse primer as a primer, but also without additional labeled probes as in the conventional manner, The 5'-cleavage reaction of the template dependent nucleic acid polymerase with 'to 3' nuclease activity can provide a detection signal of the target nucleic acid sequence. Accordingly, the present invention can achieve the object of amplifying the target nucleic acid sequence and real-time detection of the target nucleic acid sequence even using only the THD primer and the reverse primer. |
| priorityDate | 2009-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 332.