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filingDate 2009-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_695f22590c80e5689762965b2053fcc0
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publicationDate 2011-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-20110021369-A
titleOfInvention Composition for detection of m. tuberculosis complex and mycobacteria genus and simultaneous detection method for m. tuberculosis complex and mycobacteria genus with multiplex real time pcr using the same
abstract PURPOSE: A composition for detecting Mycobacteria and tubercle bacillus is provided to detect Mycobacteria and tubercle bacillus and to quickly and easily perform clinical diagnosis. CONSTITUTION: A composition for detecting Mycobacteria and tubercle bacillus contains one or more primers of sequence number 1 and 2; and one or more primers among sequence numbers 3-11. The composition further contains an internal control primer. The internal control primer is specific to plant-derived gene. A method for analyzing Mycobacteria and tubercle bacillus using the composition comprises: a step of mixing DNA from a sample and the composition for preparing a gene analysis sample; a step of performing real time PCR to obtain PCR product; a step of performing quantitation of the PCR product to obtain a quantitative curve; and a step of performing quantitation of IS6110 gene, ITS gene, and internal control gene-specific gene.
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