| abstract |
The present invention relates to a method for the simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol using recombinant E. coli,n n n To convert glycerol to 3-hydroxypropionaldehyde (3-HPA), which is a precursor of 3-hydroxypropionic acid and 1,3-propanediol , Lactobacillus glycerol dehydratase from Lactobacillus brevis derived from the start codons TTG and GTG of the open reading frame encoding DHAB1 and DHAB2 in the subunits DHAB1, DHAB2 and DHAB3 constituting the glycerol dehydratase derived from brevis ), respectively. (glycerol dehydratase; DHAB) and glycerol dehydratase reactivase from Lactobacillus brevis is transformed to be expressed; Transformed to express an enzyme that converts 3-hydroxypropionaldehyde to 3-hydroxypropionic acid; It provides a method for the simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol, characterized in that the recombinant Escherichia coli is cultured through fed-batch cultivation continuously supplying glycerol and glucose to the medium. |