| abstract |
As a primer set for amplifying a target region including a target region of detection in a NAT2 gene by gene amplification, a primer set capable of specifically amplifying the region is provided. A set of three pairs of primers comprising a forward primer consisting of the nucleotide sequences of SEQ ID NO: 7, SEQ ID NO: 33 and SEQ ID NO: 60, and a reverse primer consisting of the nucleotide sequences of SEQ ID NO: 18, SEQ ID NO: 48, and SEQ ID NO: 81, respectively . By using this primer set, it is possible to amplify three regions each containing a site where three types of polymorphisms (NAT2 * 5, NAT2 * 6, NAT2 * 7) of the NAT2 gene occur simultaneously in the same reaction solution. |