| abstract |
The present invention discloses a polynucleotide comprising at least the last six nucleotides of one of the primer sequences of SEQ ID NOS: 3-16, 18, 20-33, 35, or 37-39 or complementary to them. Also disclosed is a method of detecting the presence of a mutation in a PIK3CA gene, wherein the mutation is one of H1047R, H1047L, E542K and E545K, preferably the ARMS primer is combined with a scorpion primer. |