abstract |
By the following steps, live microorganisms, damaged bacteria, VNC bacteria, and dead bacteria of the microorganisms in the test sample are detected by flow cytometry. a) treating the test sample with cells other than microorganisms present in the test sample, protein colloid particles, or an enzyme having an activity of degrading fat, b) treating the test sample with a topoisomerase inhibitor and / or DNA ruler C) treating the test sample treated in the step a) and b) with a nuclear stain, and d) detecting microorganisms in the test sample treated with the nuclear dye by flow cytometry. Process. |