http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20100080621-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_430c89d97f6839f5364904e710af9704 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2009-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_badbed9b98669d5447876b4576252a99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39935fd3359520c717001e2ba8c20b7b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a311efd9ec9ef765e5cdf1da0193a15e |
| publicationDate | 2010-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20100080621-A |
| titleOfInvention | Method for amplification of target nucleic acid sequence, method for detection of mutation by using the method, and reagents for use in the methods |
| abstract | The present invention provides a variation detection method that enables variation detection with excellent sensitivity and reliability in one reaction system. Using the primers (Xmt) and (Xwt), the target nucleic acid sequence whose base for detection is a variant is amplified with an amplification efficiency higher than that of the target nucleic acid sequence whose base for detection is a normal type. (Xmt) is a primer having a base complementary to a region containing a mutant base in the template nucleic acid and a base complementary to the variant base in the 3 'region, and (Xwt) is a region containing a normal base in the template nucleic acid Primer having a base complementary to and complementary to the normal base in the 3 'region. It is preferable that the amplification efficiency by (Xmt) with respect to the variant template nucleic acid is higher than the amplification efficiency by (Xwt) with respect to the normal template nucleic acid. And the signal value which shows the melting state of the hybridization body of the obtained amplification product and a probe is measured, and the presence or absence of the variation of the target base site is determined from the signal value change accompanying a temperature change. |
| priorityDate | 2008-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.