http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20100059969-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-51 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-515 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-21 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2812 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2866 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2896 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 |
| filingDate | 2008-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5515e6909f965f52705cecce00727879 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_afafd7338e3674616f6197de783fde45 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d39e5dd07e2eec8351d0451c0b619fa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf1c8851e299c7404db4681c5f0e511c |
| publicationDate | 2010-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20100059969-A |
| titleOfInvention | Protein Expression from Multiple Nucleic Acids |
| abstract | The present invention discloses a method for recombinantly preparing heterologous immunoglobulins secreted from CHO cells, comprising the following steps i) to v): i) providing CHO cells adapted to grow in suspension culture, adapted to grow in serum-free medium and free of mycoplasma and virus; ii) a prokaryotic origin of replication, a first nucleic acid sequence conferring resistance to a prokaryotic selection agent, a second nucleic acid sequence encoding a heavy chain of said heterologous immunoglobulin, a third nucleic acid sequence encoding a light chain of said heterologous immunoglobulin, and a eukaryotic Providing a vector comprising a fourth nucleic acid sequence conferring resistance to a selection agent; iii) transfecting the provided CHO cells with a method comprising the following steps a) to d): a) transfecting said CHO cells with said vector comprising a fourth nucleic acid conferring resistance to a first eukaryotic selection agent, b) selecting CHO cells by growing in culture medium containing said first eukaryotic selector, c) transfecting the selected CHO cells with said vector comprising a fourth nucleic acid conferring resistance to a second eukaryotic selector that is different from the first eukaryotic selector, and d) selecting CHO cells by selective growth in culture medium containing a first eukaryotic selector and a second eukaryotic selector; iv) culturing the transfected CHO cells in medium in the presence of the first and eukaryotic selector and the second eukaryotic selector under conditions suitable for expression of the second nucleic acid and / or third nucleic acid; And v) recovering the secreted heterologous immunoglobulin from the culture medium. |
| priorityDate | 2007-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1271.