http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20100045494-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b33d8dab550a63c5affb589dcd8c13c6 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-19 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1024 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2008-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_deef022517e3ccd8f030458f64dacbfe |
| publicationDate | 2010-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20100045494-A |
| titleOfInvention | Custom Multisite Combination Assemblies |
| abstract | The present invention is the step of adding two or more or three or more primers to a double-stranded template polynucleotide of a single reaction mixture, wherein the primers do not overlap and each of the primers comprises one or more mutations different from the other primers, 1 At least one primer is a forward primer that can anneal to the minus strand of the template, and at least one primer is a reverse primer that can anneal to the plus strand of the template, and a polymerase extension reaction is performed to the reaction mixture. To produce a plurality of modified polynucleotides having various combinations of various mutations in a plurality of positions by a custom multisite combination assembly, comprising generating a plurality of extended modified polynucleotides from the three or more primers. New way To provide. This method can be performed without using the ligation step before transforming the cells with the extended modified polynucleotide. The plurality of extended modified polynucleotides can be treated with enzymes to disrupt the template polynucleotides prior to transformation of the cells. |
| priorityDate | 2007-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 521.