http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20090126538-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b31896b0595e5629c470c80b74b2d2e4 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate | 2008-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_355b60b840ab9cd1affb00513641779b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c0375d1f931c66b9383fc6d5f200df1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a99607714c8149bca19cb111a6a3852e |
| publicationDate | 2009-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20090126538-A |
| titleOfInvention | Nanoaqueum Iquitans plus DNA polymerase, preparation method thereof, and polymerase chain reaction (PCR) method using deoxytifi (DVTP) and uracil-DNA glycosylase (XD) |
| abstract | The present invention is a nano-aqueum Iquitans DNA polymerase ( Nanoarchaeum on the application of equitans DNA polymerase, hereinafter "Neq DNA polymerase, ") manufactured and Neq DNA polymerase for PCR (polymerase chain reaction, hereinafter referred to as" PCR ") using the novel PCR reaction buffer solution of will be. Neq DNA polymerase can amplify [lambda] -DNA up to 10 kb in the novel buffer solution of the present invention and perform PCR using deoxyutif (dUTP) and deoxyitif (dITP). The present invention also relates to the preparation of Neq plus (DNA) polymerase. Neq plus DNA polymerase is a Taq to Neq DNA polymerase It was prepared by mixing DNA polymerase, which can amplify λ-DNA fragments of 20 kb or more by PCR, and perform PCR 2-3 times more effectively than using Taq DNA polymerase even in the presence of deoxyutif (dUTP). Can be done. Also Taq It has a higher fidelity than DNA polymerase. In addition, the present invention is more useful than Taq DNA polymerase in the technology of preventing carry-over contamination of nucleic acid samples using deoxyutypi (dUTP) together with uracil-DNA glycosylase (UDG). Will be able to be used. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101105271-B1 |
| priorityDate | 2008-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 252.