| abstract |
Compositions and methods are provided for improving expression and / or secretion of a protein or polypeptide of interest in a host cell. A composition is provided that comprises a coding sequence for a bacterial secretion signal peptide. The coding sequence can be used in a vector construct or expression system for the transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the present invention are useful for increasing the accumulation of properly processed proteins in the periplasm of the host cell or for increasing the secretion of properly processed proteins from the host cell. In particular, secretory signal peptides include protein disulfide isomerase C (dsbC), mutant phosphate binding protein (pbp *), protein disulfide isomerase A (dsbA), CupA2, CupB2, CupC2, NikA, FlgI, tetratricopeptides Repeat family protein (ORF5550), toluene resistance protein (Ttg2C), or methyl water soluble chemotactic protein (ORF8124) secretion signal. |