| abstract |
Embodiments of the invention disclosed herein generally relate to anti-cocaine therapeutics. In particular, some embodiments of the present invention relate to a highly efficient, thermally stable, long-lasting cocaine esterase (CocE) mutant that can protect against the toxic and adjuvant effects of cocaine in a subject. Provided herein are mutant CocE polypeptides exhibiting heat stable esterase activity. Also provided are methods of treating cocaine-induced conditions in a subject in need thereof via administration of a mutant CocE, and methods for high speed screening of candidate esterase polypeptides.n n n n Anti-cocaine therapeutics, cocaine esterases, mutants, thermal stability, long-term persistence, screening |