| abstract |
The present invention (1) culturing the mesenchymal stem cells in cell proliferation medium; (2) inducing the cells cultured in step (1) into hepatocytes in serum medium to which the cell growth factor is added; And (3) subsequently, the cells induced in step (2) are added serum to at least one hepatocyte maturation inducing substance selected from Oncostatin M, glucagon, nicotine amide and dimethyl sulfoxide (DMSO). It relates to a method for producing umbilical cord mesenchymal stem cell-derived hepatocytes comprising the step of maturing the hepatocytes in the medium, the hepatocytes prepared by the method according to the present invention is similar to normal hepatocytes, albumin synthesis ability, ammonia removal ability, urea sum Performance, Cytokeratin 18 (CK 18) Synthesis, Cytochrome P450 1B1 Synthesis, and XBP-1 Synthesis It can be used as a cell source of the system. |